DNA Libraries,Isolation of Genes,Encode Protein,Prokaryotic Organisms,Genomic DNA,Structural Genes,Restriction Endonuclease

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	DNA Libraries - The isolation of genes that encode proteins is often the goal of genetic engineering experiments. In prokaryotic organisms, each of these structural genes have a continuous coding domain in the genomic DNA, whereas in eukaryotes coding regions (exons) of structural genes are separated by non coding regions (introns). Consequently, different cloning strategies have to be used for prokaryotic and eukaryotic genes.
In animals or plants, the desired sequence (target DNA) is frequently (presently in a very small portion), roughly less than 0.02% of the total chromosomal DNA. The problem lies in how to clone and select the targeted DNA sequence. To do this, the complete DNA of an organism is cut with a restriction endonuclease and each fragment is inserted in a vector. Then, the specific clone that carries the target DNA sequences must be identified, isolated and characterized.