PCR - Polymerase Chain Reaction & Gene Amplification,DNA,Gene Cloning,PCR,Kary Mullis,Califormia

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PCR - Polymerase Chain Reaction & Gene Amplification
Comparison of PCR and Gene Cloning
Types of PCR
Inverse PCR
Anchored PCR
Study of DNA Polymorphism Using PCR
PCR and RAPD Markers
PCR and VNTR Loci
PCR and SSR Loci
Molecular Mapping Using PCR
Sequence Tagged Microsatellite Sites (STMS) OR SSR Loci

	PCR - Polymerase Chain Reaction & Gene Amplification - The techniques of recombinant DNA and gene cloning, which permit us to obtain an unlimited supply of identical copies of a gene sequence or DNA segment, which is cloned in a prokaryotic or a eukaryotic cell with the help of a vector. This technique, discovered around 1975, proved extremely useful in all experiments of molecular biology and genetic engineering and thus became an essential tool in all molecular biology laboratories. In 1985, yet another remarkable tool to molecular biology was discovered, which is known as polymerase chain reaction (PCR) nick named now as people's choice reaction.
The methodology is so important that the prestigious journal Science published from USA considered PCR as the major scientific development of the year 1989, and had chosen Taq DNA polymerase, the enzyme used in PCR as the molecule of the year 1989. (Kary Mullis, who discovered this reaction in 1985, described in his article published in April 1990 issue of Scientific American, how he got the basic idea of this reaction, while driving through the red wood mountains of California, USA). The polymerase chain reaction is such a powerful technique that it may replace completely the gene cloning with vectors in due course of time.

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