Parameter |
PCR |
Gene cloning |
1. |
Final result |
Selective amplification of specific sequence |
Selective amplification of specific sequence |
2. |
Manipulation |
In vitro |
In vitro and in vivo |
3. |
Selectivity of the specific segment from complex DNA |
First step |
Last step |
4. |
Quantity of starting material |
Nanogram (ng) |
Microgram (m) |
5. |
Biological reagents required |
DNA polymerase
(Taq polymerase) |
Restriction enzymes, Ligase, vector. bacteria |
6. |
Automation |
Yes |
No |
7. |
Labour intensive |
No |
Yes |
8. |
Error probability |
Less |
More |
9. |
Applications |
More |
Less |
10. |
Cost |
Less |
More |
11. |
User’s skill |
Not required |
Required |
12. |
Time for a typical experiment |
Four hours |
Two to four days |
