Flask Cultures
The Carrel Flask Technique is used for the establishment of a strain from explants of tissue, because the flask has excellent optical properties for microscopic observation. There are two types of flask techniques. Thick dot cultures, allow rapid growth suitable for short term cultures, and thin dot cultureswhich can be maintained for a considerable period

Flask cultures are advantageous in that the tissue can be maintained in the flask for years. Large number of cultures can be easily prepared