Grid Method (Trowell, 1954)
Suitable wire mesh or perforated stainless steel sheet of size 25 x 25 mm is used as a grid, whose edges are bent to form four legs of about 4 mm height. Softer tissues like glands are first.
Techniques of organ culture, the grid method uses a metallic wire mesh or a perforated sheet with margins bent to form 4 mm high legs; explants are placed on a raft of lens paper, rayon acetate or millipore filter membrane.
Placed on rafts and are then kept on grids. Other tissues can be directly placed on the grids. The grids are placed in a culture chamber filled with fluid medium upto the grid. The chamber is supplied with a mixture of O2 and CO2. The modification of this method is widely used to study the growth and differentiation of adult and embryonic tissues.
