Immunopurification, Specific Antibody Antigen, Purify Antigens, Small Quantities, Types of Molecules, Therapeutic, Diagnostic, Treatment of Sepsis

Back to Home

Home >>Applied Biotechnology >> Cell and Tissue Culture Technology >>Immunopurification

Back to Home

Immunopurification

The highly specific antibody-antigen interaction is employed to purify antigens present in small quantities, from a mixture of several types of molecules

Table Same monoclonal antibodies of medical use derived from animal cell cultures

Product	Application
Anti-lipopolysaccharide	Treatment of sepsis
Murine anti-idiotype/human	B-cell lymphoma
Diagnostic
Anti-fibrin 99	Blood clot
Tem-Fab (breast)	Blood cancer
PR-356 CYT-356-in-111	Prostate adenocarcinoma

The risk of given the need to administer treatment frequently (because of the inability of immune response to these vectors is negligible. This is an important consideration adenovirus to integrate into chromosomal DNA). They also have advantage that they can accept much larger inserts(up to 35kb).

It is clear that for a wide variety of cell types, adenovirus (Ad) gives more efficient gene transfer compared with other systems, especially in vivo. Ad vectors can transfer genes to both proliferating and quiescent cells.

Following delivery, transgene expression is at a high level, but is transient , being low or undetectable in most tissues after two weeks. This is because Ad vectors do not integrate and for safety reasons are disabled for replication.