Properties of Finite and Continuous Cell Lines and Cell Strains, Morphology, Tissue, Fibroblast, Lymphocytic, Juveline

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Properties of Finite and Continuous Cell Lines and Cell Strains

	All lines	Source	Morphology	Age	Tissue	Characteristics
1	Finite Cell Line
	IMR90	Human lung	Fibroblasts	Embryonic	Normal	Infected by Human virus
	MRC5	Human lung	Fibroblasts	Embryonic	Normal	Infected by Human virus
	MRC9	Human lung	Fibroblasts	Embryonic	Normal	Infected by Human virus
	WI38	Human lung	Fibroblasts	Embryonic	Normal	Infected by Human virus
2	Continuous cell Line
	EB	Human	Lymphocytic	Juvenile	NP*	EB virus, +ve
	HeLa	Human	Epithelial	Adult	NP	G6PD Type A
	LS	Mouse	Fibroblastic	Adult	NP	Grow in L929 suspension
	P388D	Mouse	Lymphocytic	Adult	NP	Grow in suspension
	S180	Mouse	Fibroblastic	Adult	NP	Cancer chemotherapy
	3T3A	Mouse	Fibroblastic	Embryonic	Normal	Readily transformed

*NP Neoplastic tissue

Freeze preservation of animal cells is now a routine in all cell line banks. A cryopreservative agent like DMSO or glycerol is generally added to minimize injury to cells during freezing and thawing. Frozen ampoules are generally stored in liquid nitrogen refrigerators which are convenient and safe. Most animal cells can be stored for quite long periods, if appropriate protocols are followed.