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Suspension Culture
Here, good results depend on optimal physical and chemical factors. To keep the cells in suspension and also to make the culture homogeneous, the medium must be suitably stirred. Stirrers range from simple magnetic stirrers, flat-blade turbine impelle Grid Method rs and marine impellers to those using pneumatic energy. e.g. airlift fermenter. Improved mixing can be achieved by changing the design of the stirrer paddle or by using multiple impellers. Stirrers mix the culture without causing damage to the cells

 

Efficient aeration is achieved by bubbling air through the medium (sparging). This may damage animal cells due to high surface energy of the bubble on the cell membrane. Damage can, however, be reduced by using larger bubbles, lower gassing rates and addition of non-nutritional supplements like pluronic F-68 (polyglycol), which protect the cells from damage due to shear forces and bubbles.

The reactors used for large-scale suspension cultures are of 3 main types.
1. stirred bioreactors
2. continuous flow reactors
3. airlift fermentors

Porous microcarriers Small beads of gelatin, collagen, glass or cellulose, have a network of interconnecting pores. These provide tremendous enhancement in surface area/volume ratio and permit efficient diffusion of medium and product. These are suitable for scaling up and are equally useful for suspension and monolayer cultures.