Techniques Obtaining Cells, Disaggregation Tissues, Adherent cells, Adherent Cells, Viable Cells, Sodium Metrizoate, Ficoll

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	Techniques of Obtaining Cells by Disaggregation Of Tissues Cell cultures are generally started from disaggregated explants. Sometimes cells migrate out of the cultured whole tissues. These cells are then separated by trypsin or other enzymatic treatment to establish cell cultures. But generally, the tissues are first disaggregated either mechanically or using enzymes or chemicals so that the cell suspension could be obtained. The cells in suspension grow, to produce the primary culture.
Physical (Mechanical) Disaggregation Figure 2.1 gives a diagrammatic representation of physical disaggregation of tissues. After careful removal from the spot, the tissue is aseptically kept in a sieve of 100 mm mesh size (Figure 2.2a). The sieve is put in a sterile petri dish containing buffered medium with Balanced Salt Solution (BSS). The process of sieving is repeated to get more disaggregation of cells (Figure 2.2b). The debris remaining in the sieve are discarded and the medium containing cells is collected. The cells are counted using haemocytometer. The medium is diluted with serum to lower the level of cells to 104 cells / ml.