Advantages of Three Different Mapping Techniques -

The existence of three different mapping techniques has brought about great advances in understanding gene organization in bacteria. To map a newly discovered gene in E. coli a good approach would be to first perform an interrupted mating experiment, which positions the gene with an accuracy of about 2 minutes, equivalent to about 92 kb of DNA.

The exact position of the gene can then be established with greater accuracy by testing for co transformation or co transduction with genes known to lie in the same region of the genome, finally, the gene order can be worked out by co transduction and co transformation.