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Yeast Vectors - Yeast Cloning Plasmid Vectors - The analysis of eukaryotic DNA sequence has been facilitated by cloning the genes in prokaryotes. But some functions such as glycosylation, mitosis, meiosis, etc. are absent in prokaryotes. When genes functionally related to such a function are to be analysed, those genes have to be cloned in a eukaryotic system.

Yeast system offers the best system to study, as it is small, and easy to grow and manipulate. In addition, the biochemistry and regulation of yeast is very much like that of higher eukaryotes.

Thus yeast can be a very good surrogate host like E. coli, for studying the structure and functions of eukaryotic gene products. Various yeast vectors have been designed, once the ability and utility of yeast is confirmed. All of them have three features in common.

1. All of them contain unique target sites for a number of RE.

2. All of them can replicate in E.coli of ten at high copy number.

3. All of them employ markers that can be used to select recombinant yeasts, e.g. Hi53, leo2, trpl and ura3.

 

  

 
 

 
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