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Hydrogen Gas Using Hydrogenase.

Some microbes possess the enzyme hydrogenase, which helps the two electrons join who hydrogen ions to produce one molecule of hydrogen gas (H2).

These microbes include a number of algae (e.g. Scenedsmus, Chalamydomonas, Dunaliella, Porphyridium and Oscillatoria) and some photosynthetic bacteria (e.g. Rhodospirillium, Chromatium, Clostridum and Thiocapsa), which can be gainfully employed for hydrogen production, although their commercial exploitation is still at a very early stage of development.

The above microbes may be used for the isolation of hydrogenase enzyme which can be used with isolated chloroplasts. The hydrogen ions (H+) and electrons liberated due to the use of solar energy by the chloroplasts are made to combine into hydrogen molecules,

which will bubble out of the solution and can be easily collected. This system though presently works only for a few hours, but can be substantially improved to become commercially successful.
   

 
   
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