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Diagnosis of Diseases Caused by Protozoa and Helminths -  In order to supplement the conventional methods available for diagnosis, of different diseases, biotechnology has provided monoclonal antibodies and DNA probes as two very effective and most sensitive tools. Monoclonal antibodies and DNA probes are being prepared and made available for the diagnosis of a variety of diseases.

Monoclonal antibodies can be used through serological tests, which will take only minutes, while the conventional methods may sometimes take weeks, since they may require culturing of bacteria or viruses as in case of herpes virus or other viruses.

Similarly, DNA probes, which are even more sensitive than monoclonal antibodies, will, however, take hours (not minutes as in monoclonal antibodies) instead of weeks. Readymade DNA probes for herpes virus and other human, animal and plant viruses are being prepared.
DNA probe kits are also available to help preparation of DNA probes and a market of DNA probes for several hundred million dollars (> $500,000,000) per year is now available. This market will expand and grow in future.

The diagnostic methods at the DNA level have the advantage over other methods, since in these methods genes of the parasite are examined and not the expressed product which changes in different stages of the life cycle of the parasite and also due to the environment.

Rapid progress in recent years has been made in the development of nucleic acid based assays for the diagnosis and epidemiological surveillance of human parasites. Probes are now available for a number of human parasites from the group Protozoa and Helminths (both Platyhelminths and Nematyhelminths).

In India also, recently (1986) a diagnostic probe for the detection of malaria has been constructed at the Astra Research Centre India (ARCI), which is established as a joint venture between India and AB Astra, Sweden, (inaugurated by Prime Minister, Rajiv Gandhi on Jan. 7, 1987). Methods for the detection of several other diseases are also being developed at this centre.

In the above use of DNA probes, DNA has to be radioactively labeled, which is not very safe in field study. Therefore, techniques for non radioactive labelling of probes, methods for quick preparation of target DNA and suitable protocols for hybridization are being developed. This in future will allow quick diagnosis of parasites in the field.