| No |
Parasite |
Probe used |
Hybridization technique used |
| |
I.Protozoa |
|
|
| 1. |
Leishmania (kala-azar) |
Kinetoplast DNA (kDNA. a repetitive DNA sequence) |
Dot blots, Southern blots, in, situ hybridization |
| 2. |
Trypanosoma (sleeping sickness) |
Kinetoplast DNA (kDNA); a repetitive DNA and total parasite DNA |
Southern blot (restriction fragment length polymorphism = RFLP) |
| 3. |
Plasmodium (malarian parasite; P. falciparum,P. vivax and p.cynomolgi) |
Repeated DNA; synthetic oligonucleotide |
Dot blots; blood samples lysed directly on nitrocellulose filters |
| |
II. Helminths |
|
|
| 1. |
Schistosomes (human blood flukes) |
Ribosomal RNA gene |
Dot blots; Southern blots |
| 2. |
Namatodes:Wuchereria and Brugia (filaria);Onchocerca ()river blindness); Trichinella |
Repetitive DNA sequences; synthetic oligonucleotides;
ribosomal DNA; |
Crude DNA, blood samples: dot blots
|
| 3. |
Teanilid cestodes ()Taenia solium, etc |
Ribosomal DNA; |
Southern blots |
