Technology for Hybrid Seed Production, Different Strategy, Utilizing, Terminator Recombinase, Repressor gene, Parental Lines Contains

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Terminator and Traitor Technologies
Terminator and Traitor Technologies Introduction
Terminator Technology the v-GURT
The Patent and its Possible Uses
Hybrid Varieties vs Terminator Technology and IPRs
Mechanism involved in Terminator Technology
Technology for pure Lines Seed Production
Technology for Hybrid Seed Production
Verminator an Alternative to Terminator
Defence by D and PL/Monsantp

Technology for hybrid seed production

In case of hybrid seed production, a different strategy, utilizing only two genes (terminator and recombinase), one in each of the two parents of the hybrid are used and no repressor gene is needed.

One of the parental lines contains the recombinase gene, which becomes active only after germination, and the other parent contains the lethal (terminator) gene separated from its promoter by Ii spacer (blocking sequence).

Genetic Basis of Terminator Technology

A. Terminator Gene B. Recombinase Gene

The hybrid progeny, which is the technology protected hybrid seed bought and planted by the farmer, thus contains both the elements of the system in every cell.

The recombinase, expressed right after germination, excises the spacer blocking sequence bringing the promoter and lethal gene together. Since the promoter is embryo specific, the lethal gene does not express till seed development starts.

During seed development, the lethal gene expresses during late embryogenesis and kills the embryo. Thus the seeds harvested from the first generation hybrid crop will be normal in all essential respects, except that they will not germinate if sown as a crop.