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Technology for pure lines seed production

In self-pollinated crops, where pure lines are used as cultivars, the promoter for the above recombinase gene is repressor specific and is used by a specific repressor protein encoded by the third gene, which is a repressor gene.

The repressor gene can be switched off using a stimulus in the form of a chemical, a heat shock, etc. Due to the stimulus, the repressor gene is switched off and Cre/Lox (recombinase gene) is switched on.

This will lead to the production of recombinase enzyme, which will excise the blocking sequence or the spacer which blocked the expression of the terminator gene (e.g. RIP).

Due to excision of the blocking sequence, the promoter will come to lie adjacent to the terminator gene, which will now be expressed and will kill the developing embryos. In actual commercial production and sale of seeds of pure lines as above, the crop for commercial seed production will be grown by the breeder or seed company without chemical treatment.

The harvested seeds from this crop, will be treated with the chemical, before it is sold to the fanner, so that in crops grown by the farmer, the toxic substance is produced at the time of embryo development, and the embryos die or fail to develop.

Genetic Basis of Terminator Technology

At Breeders Field viable seed with embryo and endosperm is produced, Genetic basis of terminator technology envisaged for pure line seed production in self pollinated crops
Genetic basis of terminator technology envisaged for pure line seed production in self pollinated crops
Genetic basis of terminator technology envisaged for pure line seed production in self pollinated crops
Genetic basis of terminator technology envisaged for pure line seed production in self pollinated crops


A seed thus produced will carry the endosperm, but not the embryo, so that it can be used or sold as grain, but cannot be used for sowing.

Tetracycline
is one such chemical trigger that can be used for treatment of the seeds before selling them in the market.