Applications of Blotting and Hybridization Techniques -
1. Southern blotting technique is widely used to find specific nucleic acid sequence present in different animals including man. For example if we want to know whether there is a gene like insulin in sea anemone, then DNA of sea anemone is mobilized on membrane and blotted by using insulin probes against it.

2. Northern blotting technique is widely used to find gene expression and regulation of specific genes. For example if we find human insulin like gene in oyster, then by isolating and immobilizing RNA and blotting it with insulin probe we call tell whether the gene is expressing or not.

3. By using blotting technique we can identify infectious agents present in the sample.

4 . We can identify inherited disease.
5. It can be applied to mapping restriction sites in single copy gene.