Western Blotting Techniques - In western blotting, proteins are transferred from the polyacrylamide gel (PAGE) or sodium dodecyl sulphate PAGE onto the nitrocellulose membrane or nylon membrane. In this method there is no requirement for pre-treatment as the proteins are small and in most cases they are not linear. The second difference is that there is no need for cross linking of the proteins to the membrane.

After the transfer of proteins from the gel onto the membrane, it is incubated with or in a solution containing antibodies against protein of interest. Non-bound antibodies are washed off the membrane and the presence of the initial antibody is detected by placing the membrane in a solution containing a secondary antibody.