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Mapped Chloroplast Genes with Chloroplast DNA

Ribosomal RNAs: Ribosomal RNA operons are designated rrnA, B,C and so forth, depending on the number of gene sets in the particular genome. In most chloroplasts there is one operon in each segment of the inverted repeat. These are normally identical in sequence. By convention, rrnA is on the right side of the chloroplast DNA restriction map with the large single copy region at the top, The rbcl gene is on the left side. Each operon normally includes genes for 16S rRNA, 23S rRNA, 5S rRNA, and 4.55 rRNA.

Transfer RNAs: tRNA genes are designated "trn' to indicate transfer RNA, followed by the single letter amino acid code indicating the amino acid accepted by the tRNA encoded by the gene. Where there is more than one gene for a particular amino acid, the isoaccepting species can be indicated either with sequential numbers or by giving the anticodon. About 40 tRNA genes are known to exist in the chloroplast genome. Examples:

trnF-gene for tRNA(Phe)
trnC-gene for tRNA(Cys)
trn L1 (or trnL-UAA)--gene for tRNA(Leu)1
trn L2 (or trnL-CAA)--gene for tRNA(Leu)2

Ribosomal Proteins

rps 4-ribosomal protein homologous to E. coli ribosomal protein S4.
rps 19---ribosomal protein homologous to E. coli ribosomal protein S 19
rpl 2-ribosomal protein homologous to E. coli ribosomal protein L2.

Photosystem I Proteins
psaA 1-P700 chlorophyll a apoprotein
psaA2-P700 chlorophyll a apoprotein

Photosystem II Proteins
psbA-"32 kilodalton' quinone-binding polypeptide. Also known as "photogene 32" and "Qb protein"; it contains the binding site for atrazine type herbicides.
psB-51 kilodalton chlorophyll a-binding polypeptide or p680 apoprotein.
pbC-44 kilodalton chlorophyll a-binding polypeptide.
pbD-"D2" protein.
psbE-cytochrome b559.
Photosynthetic Electron Proteins
pet-cytochrome f
petS-cytochrome b6
petD-subunit 4 of the cytochrome b6/f complex

Proteins of the ATP Synthase Complex
atpA-CF1 alpha subunit
atpS-CF1 beta subunit
atpE-CF1 epsilon subunit
atpH-CFO subunit III, DCCD-binding proteolipid for the ATPase complex (proton translocating subunit)

Carbon Fixation Enzymes
rbcL-ribulose bisphosphate carboxylase, large subunit
Other Stromal Polypeptides
tufA-translational elongation factor Tu