F-Factor Based Vectors

F-Factor Based Vectors - F- factor based vectors have recently been developed for cloning large DNA segments (125 kbp) in E. coli. The cloning of large DNA segments is achieved by a method called chromosomal building, in which through repeated recombination, size of cloned segment can be increased.

These bacterial vectors will complement the YAC vectors for cloning segments larger than 100 kbp in length and offers some advantages over YAC system. These vectors have already been used for cloning large DNA segments from the bithorax gene of Drosophila (for details consult Science; 16 June, 1989).