PI Cloning Vectors for Cloning Large DNA Segments

PI Cloning Vectors for Cloning Large DNA Segments - The bacteriophage PI cloning vectors can allow cloning of 100 kbp long DNA segments with an efficiency of 105 clones per fig of insert DNA.

Therefore, in their capability, they fall between YACs and cosmids. The vector with insert DNA is amplified in E. coli and several micrograms of cloned DNA can be recovered from 5-10 ml of exponential phase of E. coli cells.