Animal Viruses

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  Cloning and Expression Vectors

  Cloning Vectors for Recombinant DNA

  Plasmids as Vectors

 pBR322 and pBR327 Vectors

 pUC Vectors

  Yeast Plasmid Vectors

  Examples of Yeast Plasmid Vectors

  Retriever Vectors

  Cosmids as Vectors

  Phagemids as Vectors

  Bluescript II KS

  PI Cloning Vectors for Cloning Large DNA Segments

  F-factor Based Vectors

  Plant and Animal Viruses as Vectors

  Plant Viruses ( Cauliflower Mosaic Virus OR CaMV and Geminivirus

  Important Geminiviruses

  Animal Viruses

  Transposons as Vectors

  Artificial Chromosome ( YAC and MAC) Vectors for Cloning Large DNA Segments

 Expression Vectors for High Level of Expression of Cloned Genes

  Promoters

  Nopaline Synthase (nos) Promoter from T-DNA

  Dual Promoter of Mannopine Synthase (mas) Genes 1 and 2

  35S RNA Promoter of CaMV

  Polyhedrin Promoter from Baculovirus

  Expression Cassettes

  Baculovirus and Expression Vector System for Insect Cells

  Virus Expression Vector for Mammalian Cells

 Transforming Viruses Used as Vectors in Mammalian Cells

  Binary and Shuttle Vectors

 

Animal Viruses

Animal Viruses - A number of animal viruses are also used as vectors, either for the delivery of nucleic acid into cultured cells followed by its integration with host genome or for the amplification and high level expression of foreign gene, using the promoters from virus genes.

These cloned genes can have a variety of uses including gene therapy in mammalian cells and synthesis of important proteins by cloned genes in cell cultures. Some of these viruses will be discussed later in this, because they are used as expression vectors

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  Ti and Ri Plasmids as Vectors for Higher Plants

  Bacteriophage as Vectors

  Lambda Phage Vectors

  EMBL3 and EMBL4 (Replacement Vectors)

 Charon 34 and Charon 35

  M13 Phage as Vector for DNA Sequencing