Charon 34 and Charon 35 - Charon 34 and Charon 35 differ from each other only in their central fragments and will accept fragments 9-20 kb (kilobases) long. They have more extensive range of restriction targets within their polylinkers than do EMBL3 and EMBL4.

In these vectors, physical separation of lambda arms from central fragment (stuffer) is required before ligation to donor fragments. In EMBL3 and EMBL4, removal of central fragment is not required, because there is a mechanism which ensures that 98% of the packagable DNA are recombinants