Cloning Vectors for Recombinant DNA

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  Cloning and Expression Vectors

  Cloning Vectors for Recombinant DNA

  Plasmids as Vectors

 pBR322 and pBR327 Vectors

 pUC Vectors

  Yeast Plasmid Vectors

  Examples of Yeast Plasmid Vectors

  Retriever Vectors

  Cosmids as Vectors

  Phagemids as Vectors

  Bluescript II KS

  PI Cloning Vectors for Cloning Large DNA Segments

  F-factor Based Vectors

  Plant and Animal Viruses as Vectors

  Plant Viruses ( Cauliflower Mosaic Virus OR CaMV and Geminivirus

  Important Geminiviruses

  Animal Viruses

  Transposons as Vectors

  Artificial Chromosome ( YAC and MAC) Vectors for Cloning Large DNA Segments

 Expression Vectors for High Level of Expression of Cloned Genes

  Promoters

  Nopaline Synthase (nos) Promoter from T-DNA

  Dual Promoter of Mannopine Synthase (mas) Genes 1 and 2

  35S RNA Promoter of CaMV

  Polyhedrin Promoter from Baculovirus

  Expression Cassettes

  Baculovirus and Expression Vector System for Insect Cells

  Virus Expression Vector for Mammalian Cells

 Transforming Viruses Used as Vectors in Mammalian Cells

  Binary and Shuttle Vectors

 

Cloning Vectors for Recombinant DNA

Cloning Vectors for Recombinant DNA - One of the most important uses of recombinant DNA technology is the cloning of

(i) random DNA or cDNA segments, often used as probes or

(ii) specific genes, which may be either isolated from the genome or synthesized organochemically or in the form of cDNA from mRNA. This cloning of DNA is possible only with the help of another DNA molecule, which is capable of replicating in a host.

This other DNA molecule is often used in the form of a vector, which could be a plasmid, a bacteriophage, a derived cosmid or phagemid, a transposon or even a virus. Techniques should also be available, which will allow selection of chimeric genomes obtained after insertion of foreign DNA from a mixture of chimeric and the original vector (see later).

Another critical desired feature of any cloning vector is that it should possess a site at which foreign DNA can be inserted without disrupting any essential function. Therefore, in each case an enzyme will also have to be selected which will cause a single break.

Sometimes vectors are modified by inserting a DNA segment to create unique site(s) for one or more enzymes to facilitate its use in gene cloning. This inserted DNA with restriction sites for several enzymes is sometimes called a polylinker.

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  Ti and Ri Plasmids as Vectors for Higher Plants

  Bacteriophage as Vectors

  Lambda Phage Vectors

  EMBL3 and EMBL4 (Replacement Vectors)

 Charon 34 and Charon 35

  M13 Phage as Vector for DNA Sequencing