Examples of Yeast Plasmid Vectors

Examples of Yeast Plasmid Vectors - The possible examples of the yeast plasmid vectors are :

(i) YIp or yeast integrative plasmids, which allow transformation by crossing over and have no replication origin

(ii) YEp or yeast episomal plasmids, which carry 2 micron DNA sequence including the origin of replication and rep genes

(iii)YRp or yeast replicating plasmids, which carry autonomously replicating sequence; this sequence is very common with many yeast genes so that stable transformation can be achieved by crossing over

(iv)YCp or yeast centromere (CEN) containing plasmids, which function as true chromosomes and segregate during mitosis and meiosis

(v)pYAC or yeast artificial yeast chromosome vector which carries both centromere and telomere sequences and, therefore, can be used to obtain artificial chromosomes.

The above vectors have been extensively utilized for a study of yeast genome and are also used as shuttle vectors, which allow genic sequences to be routinely transferred back and forth between yeast and E. coli cells, provided they contain origins of replication active in both yeast and E. coli.

These vectors also have markers enabling the selection of E. coli cells or colonies transformed with these vectors. The vectors can therefore be amplified in E. coli and then used to transform yeast cells