Expression
of
Vectors
for
High
Level
of
Expression
of
Cloned
Genes - In a number of cloning vectors described earlier in this chapter, the main utility has been to clone a gene or a DNA sequence. In other words, they are used for obtaining millions of copies of the cloned DNA segment, for further use in either the genetic engineering experiments or for further basic studies.
With this objective in mind, the cloned genes in these vectors need not express themselves either at the transcription level (production of mRNA) or at the translation level (production of protein).
