Plant and Animal Viruses as Vectors

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  Cloning and Expression Vectors

  Cloning Vectors for Recombinant DNA

  Plasmids as Vectors

 pBR322 and pBR327 Vectors

 pUC Vectors

  Yeast Plasmid Vectors

  Examples of Yeast Plasmid Vectors

  Retriever Vectors

  Cosmids as Vectors

  Phagemids as Vectors

  Bluescript II KS

  PI Cloning Vectors for Cloning Large DNA Segments

  F-factor Based Vectors

  Plant and Animal Viruses as Vectors

  Plant Viruses ( Cauliflower Mosaic Virus OR CaMV and Geminivirus

  Important Geminiviruses

  Animal Viruses

  Transposons as Vectors

  Artificial Chromosome ( YAC and MAC) Vectors for Cloning Large DNA Segments

 Expression Vectors for High Level of Expression of Cloned Genes

  Promoters

  Nopaline Synthase (nos) Promoter from T-DNA

  Dual Promoter of Mannopine Synthase (mas) Genes 1 and 2

  35S RNA Promoter of CaMV

  Polyhedrin Promoter from Baculovirus

  Expression Cassettes

  Baculovirus and Expression Vector System for Insect Cells

  Virus Expression Vector for Mammalian Cells

 Transforming Viruses Used as Vectors in Mammalian Cells

  Binary and Shuttle Vectors

 

Plant and Animal Viruses as Vectors

Plant and Animal Viruses as Vectors - A number of plant and animal viruses have also been used as vectors both for introducing foreign genes into cells and for gene amplification and expression in host cells. In the latter case, we may be interested only in getting increased quantity of the gene product and may not be interested in the integration of the foreign gene in the host genome. Some of the viruses that are commonly used as vectors will be described in this section.

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  Ti and Ri Plasmids as Vectors for Higher Plants

  Bacteriophage as Vectors

  Lambda Phage Vectors

  EMBL3 and EMBL4 (Replacement Vectors)

 Charon 34 and Charon 35

  M13 Phage as Vector for DNA Sequencing