Transposons as Vectors

     BACK TO HOME

  Cloning and Expression Vectors

  Cloning Vectors for Recombinant DNA

  Plasmids as Vectors

 pBR322 and pBR327 Vectors

 pUC Vectors

  Yeast Plasmid Vectors

  Examples of Yeast Plasmid Vectors

  Retriever Vectors

  Cosmids as Vectors

  Phagemids as Vectors

  Bluescript II KS

  PI Cloning Vectors for Cloning Large DNA Segments

  F-factor Based Vectors

  Plant and Animal Viruses as Vectors

  Plant Viruses ( Cauliflower Mosaic Virus OR CaMV and Geminivirus

  Important Geminiviruses

  Animal Viruses

  Transposons as Vectors

  Artificial Chromosome ( YAC and MAC) Vectors for Cloning Large DNA Segments

 Expression Vectors for High Level of Expression of Cloned Genes

  Promoters

  Nopaline Synthase (nos) Promoter from T-DNA

  Dual Promoter of Mannopine Synthase (mas) Genes 1 and 2

  35S RNA Promoter of CaMV

  Polyhedrin Promoter from Baculovirus

  Expression Cassettes

  Baculovirus and Expression Vector System for Insect Cells

  Virus Expression Vector for Mammalian Cells

 Transforming Viruses Used as Vectors in Mammalian Cells

  Binary and Shuttle Vectors

 

Transposons as Vectors

Transposons as Vectors - Transposons of higher plants (Ds, Ac or Mu 1 of maize). Ac and Ds are popular transposons in corn, and were earlier known to represent activator dissociation (Ac-Ds) system. Each represents a transposon with short terminal repeats enclosing a long DNA segment, which measures more than 4500 bp in Ac and about 400 bp in Ds.

Each possesses genes including the gene for transposase enzyme responsible for transposition. Part of this region can be deleted and the transposon can be used for cloning of foreign DNA segment in much the same way as in other cases.Transposons of Drosophila (P elements)

P element of Drosophila consists mainly of 31 bp (base pairs) terminal inverted repeats enclosing a 3 kilobase protein coding region, coding for transposase and a repressor of transposition. Many internally deleted P elements are also found, where the transposase derived from other complete P elements help their mobilization.

In these deleted. P elements, gene for transfer can be inserted to yield recombinant P element, which can be microinjected into the fertilized egg, along with normal P element (helper P to supply transposase).The inserted gene is transposed onto an embryonic chromosome

     BACK TO HOME

  Ti and Ri Plasmids as Vectors for Higher Plants

  Bacteriophage as Vectors

  Lambda Phage Vectors

  EMBL3 and EMBL4 (Replacement Vectors)

 Charon 34 and Charon 35

  M13 Phage as Vector for DNA Sequencing