Culture Media Preparation
-Dry powdered media having required concentration of different constituents are now available commercially, so that this powder is dissolved in distilled water (10% less than the final volume). Agar, sugar and other desired supplements are added and the final volume is made up using distilled water. The pH is then adjusted (5.0 - 6.0) and medium is autoclaved. When standard medium is not satisfactory, the required quantities of different components are weighed and dissolved.

The medium prepared as above is poured into the desired culture vessels (15 ml for 25 x 150 mm culture tubes; 50ml for 150 ml flasks, which are plugged with non absorbent cotton wool wrapped in cheese cloth).