Culture of Single Cells (Bergmanns Cell Plating Techniques) - In this technique, free cells are suspended in a liquid medium (if cell aggregates are there, it is filtered). Culture medium with agar (0.6 - 1%) is cooled and maintained at 35°C in a water bath.

Equal volumes of liquid and agar media are mixed and rapidly spread in a Petri dish, so that cells are evenly distributed in a thin layer, after solidification. The Petri dishes are sealed with parafilm and examined with inverted microscope to mark single cells (marking is done on outer surface of the dish).