Liposome Mediated DNA Library - The system currently receiving the most attention and considered to hold the most promise involves the use of liposomes.

These are small artificial lipid vesicles prepared from phosphatidyl choline and stearylamine by a process known as reverse phase evaporation.

Nucleic acid entrapped in such liposomes renders them highly tolerant to attack by nucleases.

Techniques for fusing these liposomes to plant cell protoplasts have been evolved.

Possible advantages perceived in using liposomes as a delivery system include:

Virus production could also be detected following incubations with neutral or positively changed liposome preparations.

The same thing has been done with Petunia also. It is likely that liposomes will have their greatest application in transformation experiments utilizing non- Ti plasmid vectors and in experiments with protoplasts which are outside the host range of A. tumefaciens.

Uniform artificial lipid vesicles of 0.4 μm or more in diameter can be made by sonicating a mixture of phosphatidyl serine and an aqueous buffer. DNA or chromosomes were encapsulated in these large unilamellar vesicles.