Antibiotic Production- Penicillin amidases (= penicillin acylases) obtained from E. coli are immobilized in a variety of ways, e.g., by covalent linkage to cyanogen bromide activated Sephadex 200. It is used in a batch or semi continuous mode STR, and may be used 100 times.

In a PBR, it has been used for 100 days yielding about 2 tonSj1tPf 6-aminopenicillonic acid/kg of immobilized enzyme. Penicillin G amidases may be used to synthesize penicillin and cephalosporins.