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Restriction Enzymes - DNases which act on specific positions or sequences on the DNA are called as restriction endonucleases.

The sequences which are recognized by the restriction endonucleases or restriction enzymes (RE) are called as recognition sequences or restriction sites.

These sequences are palindromic sequences (i.e., if read from right to left, the sequence is same, e.g. MADAM).

Different restriction enzymes present in different bacteria can recognize different or same restriction sites. But they will cut at two different points within the restriction site. Such restriction enzymes are called as isoschizomers. Interestingly no two restriction enzymes from a single bacterium will cut at the same restriction site