Type II Restriction Enzymes - Cohesive End Cutters - Cohesive end cutter Type II restriction enzymes of this class cut the DNA stands at different points on both the strands of DNA within the recognition sequence.

They generate a short single-stranded sequence at the end.

This short single strand sequence is called as sticky or cohesive end. This cohesive end may contain 5 -PO 4 or 3 -OH, based upon the terminal molecule (5 -PO4 or 3 -OH).

These enzymes are further classified as 5end cutter (if 5 -PO 4 is present) or 3 -end cutter (if3' -OH is present).