Anaerobic Bacteria, Enzyme Hydrogenase, Species of Bacteria, Cellulose

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	Anaerobic Bacteria-Anaerobic bacteria oxidise the substrate by reducing NAD⁺ to NADH. But for a continued substrate oxidation it is essential to remove NADH from the cell environment and regenerate NAD⁺. The electrons from NADH are transferred to H⁺ ions to produce H₂ gas, thereby regenerating NAD⁺; the reaction is catalyzed by the enzyme hydrogenase.
If H₂ producing bacteria are grown in the absence of H⁺ utilizing species of bacteria, H₂ accumulates and can be collected. The substrate for such a digestion can be any biodegradable organic material, including cellulose that has first been hydrolyzed.