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Simple Gene - A continuous sequence in a nucleotide that codes for a specified polypeptide or functional RNA is called as simple gene.

The functional portion of the gene obviously is the sequence of nucleotides that encode for the protein/amino acid that plays a role in the metabolic process of the cell.

As RNA sequence is complimentary to the coding strand of DNA, it is customary to represent a gene by complimentary strand sequence of duplex DNA.

Transcriptional unit is a sequence of DNA transcribed into a single RNA, starting at the promoter and ending at the terminator.

In prokaryotes, a transcriptional unit is continuous but in the case of eukaryotes, we find intergenic spacers.

Intergenic spacers are the genomic regions that are not directly involved in the final product and are found between actual coding sectors of two adjacent genes.

Spacers are not introns. Spacers are classified into two types based upon their location leader (present at the 5 end of the starting of gene) and train (present at the 3 end of the gene).