Back to Home
Home >> Gene Transfer Methods in Plants >> Agroinfection and Gene Transfer
Back to Home

Agroinfection and Gene Transfer - Agroinfection is a phenomenon, in which a virus infects a host as a part of T-DNA of Ti plasmid carried by Agrobacterium. Viral DNA can be integrated into the T-DNA and can be delivered into plant cells with the normal Agrobacterium T-DNA transfer process. After infection viral DNA is released to form functional virus that replicates and spreads systemically.

Agroinfection may also lead to the integration of viral DNA so that transgenic plant containing integrated viral DNA can be produced.In maize, agroinfection with maize streak virus has been demonstrated.

This suggested that Agrobacterium based vector system can be used for genetic engineering in cereals, although ordinarily Agrobacterium does not infect monocotyledons. Thus agroinfection can lead to the production of transgenic plants, even though it has no better chances of yielding transgenic cereals than does Agrobacterium infection alone.

However, agroinfection has great potential for studies in virus biology, because it can transfer deletion mutations or even single viral genes.