Routes
for
Production
of
Transgenic
Plants
Target cell type |
Method used for regeneration |
1. Cultured cells or protoplasts |
Organogenesis or embryogenesis via callus phase |
| 2. Meristem cells from immature embryo or organ | In vitroplant regeneration from transformed cells. |
| 3. Cells in immature embryos shoot and flower meristem | Normal development (in vivo)of embryo, shoot or flower followed by use of pollen (transformed) from chimaeric plant to produce transformed seed. |
| 4. Pollen | Pollen (treated with DNA) used for pollination leading to the production of transgenic plants. |
| 5. Zygote | Direct development (in vivo)of transgenic plants. |