Transformation Techniques Using Agrobacterium -Agrobacterium infection (utilizing its plasmids as vectors) has been extensively utilized for transfer of foreign DNA into a number of dicotyledonous species. The only important species that have not responded well, are major seed legumes, even though transgenic soybean (Glycine mar) plants have been obtained.

The success in this approach for gene transfer has resulted from improvement in tissue culture technology. However, monocotyledons could not be successfully utilized for Agrobacterium mediated gene transfer except a solitary example of Asparagus.

The reasons for this are not fully understood, because T -DNA transfer does occur at the cellular level. It is possible that the failure in monocots lies in the lack of wound response of monocotyledonous cells.