Microinjection - Gene Transfer Methods - The technique of microinjection is based on the use of glass micropipettes with a 0.5-10 mm diameter tip to directly transfer macromolecules (vectors) into the cytoplasm or nucleus of a recipient cell which is held by a glass suction pipette.

This method is widely used for animal and plant cells.

In this method the recipient cell is held with a suction pipette by using suction pressure.

Micropipette filled with DNA solution or vector DNA is brought to the recipient cell.

Then an appropriate amount of DNA solution is injected into the recipient cell by applying pressure exerted by syringes or mechanized machines connected to the micropipette.