Cloning by Immunological Methods - Immunochemical detection of clones can be applied, provided the bacteria having the vector code for the protein specific of the insert. This method uses the 3 points of ability of IgG molecules for screening.

1. IgG can bind to different determinants or epitopes on the antigen.

2. It binds to plastic or PVC very strongly and cannot be removed by repeated washing.

3. IgG molecules can be radiolabelled very easily and by in vitro method.

The only drawback in this method is that it can be applied only when IgG molecules against the insert coded protein are available. In this method, transformed cells growing on the petri plate are replicaplated onto a fresh plate, as the subsequent steps will kill the cell. After replica plating, the cells are killed or lysed by using chloroform or UV.