Back to Home
Home >> Haploid Production and Uses >>Barley Haploids Through Crosses with Hordeum Bulbosum
Back to Home

Barley Haploids Through Crosses with Hordeum Bulbosum - A situation analogous to somatic reduction occurs when in a interspecific or intergeneric hybrid, chromosomes of one parental genome are preferentially eliminated.

This method has been extensively utilized for production of haploids in barley and also in wheat. The production of haploids in barley in this manner involves interspecific hybridization of Hordeum vulgare (2n = 14) with H. bulbosum (2n = 14), a cross pollinated, self incompatible perennial species. Most progeny (95%) are barley haploids, remainder being diploid hybrids. A cross between 2x barley and 4x H. bulbosum mainly produces triploid hybrids.

Cytological examination of early embryo and endosperm in VV X BB or. BB X VV hybrids revealed gradual elimination of chromosomes. While most genotypes of barley readily produce haploids, chromosome elimination seems to be controlled by genotypes of both parents.

Kasha and Kao (1970) summarized the findings for haploid production using the above technique as follows:

(i) a very high frequency of haploids can be obtained and these haploids are not associated with any diploid hybrids;

(ii) these haploids can be obtained with any cultivated barley and would resemble cultivated barley genome in reciprocal crosses. Contrary to these observations, Lange (1971) showed that haploids obtained due to chromosome elimination are associated with hybrids.

With embryo culture technique, he was able to obtain viable hybrids from several cross combinations, although frequency of hybrids was low except in case of VBB hybrids, which were quite frequent.