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Differentiation of Pollen - An anther or pollen has two distinct morphologies which have the potential to develop into two different types of generations gametophyte and sporophyte. This is referred to as pollen dimorphism.

The pollen capable of forming haploids (embryogenic pollen) is small with a cytoplasm that stains less intensely with acetocarmine than does gametophytic pollen. Such pollen is usually low in quantity and accounts for a very low frequency of haploid formation. Exposure of plants to low temperatures before flowering increases the frequency of embryogenic pollen.

The pollen mother cell is diploid and has a determinate structure. It undergoes cell reorganization for differentiating into microspore (haploid) cells which are indeterminate structures. Determination into either androgenic (gametophytic) or embryogenic (sporophytic) grains is possibly a function of differential gene activity.

Elaborate endoplasmic reticulum, abundance of ribosomes, and normal mitochondria are usual manifestations of gametophytic pollen, whereas quiescence and repression of organelles in micro spores lead to differentiation of embryogenic pollen.

Gametophytic pollen germinates in the presence of metabolic inhibitors in contrast to embryogenic pollen; which does not germinate because of sensitivity to these antimetabolites.