Diploidization of Haploids Plants - Haploid plants obtained either from anther or ovule culture may grow normally under in vitro conditions up to the flowering stage but viable gametes are not formed due to the absence of one set of homologous chromosomes and, consequently, there is no seed set.

The only mechanism for perpetuating the haploids is by duplicating the chromosome complement in order to obtain homozygous diploids. In pollen derived plants duplication of chromosomes may occur spontaneously in cultures but due to the small percentage of such double-haploids it is necessary to diploidize the haploids by chemical means.