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Hybrid Sorting in Haploid Breeding - One of the essential steps in haploid breeding involves selection of superior plants among haploids derived from F1 hybrids through anther culture. It is popularly described as hybrid sorting and virtually means selection of recombinant superior gametes.

The haploid method of breeding involving hybrid sorting is considered superior over pedigree and bulk methods, firstly because the frequency of superior gametes is higher than the frequency of corresponding superior plants in F2 generation, and secondly because haploid breeding reduces significantly the time required for development of a new variety (see later).

For instance, if we assume that the .frequency of superior F1 gametes is one in one hundred, then the frequency of homozygous F2 plants derived from the fusion of two such superior similar gametes would be one in ten thousand. Therefore, smaller populations of doubled haploids derived from haploids will need to be handled.

The above technique of hybrid sorting has been successfully utilized, particularly in China and Japan, for development of new varieties in several crops.

In Japan, an excellent tobacco cultivar, 'F-211' bred through anther culture, is resistant to bacterial wilt and has a mild smoking quality. In China, more than 100 rice varieties developed using the same technique, gave an increased yield and, therefore, occupy significant areas of rice cultivation.

Haploids have also been successfully utilized for breeding of barley, maize, sugarcane, oilseed rape or canola (B. napus), bell peppers and some other crops.

In barley, atleast two varieties (Mingo, Gwylan) have been produced in Canada using haploids derived through bulbosum technique of chromosome elimination.

The varieties were superior for characters like disease resistance (against powdery mildew and barley yellow mosaic virus) and other yield contributing traits. Similarly, in maize superior inbred lines were developed through screening of haploids derived from anther culture in a hybrid. In sugarcane, selection among anther-culture derived haploids led to the development of superior lines with tall stem and higher sugar content.

In Brassica napus also, anther derived doubled haploids, produced in Canada, had low erucic acid and glucosinolate content. In another case, a haploid derived from twin seedlings in B. napus led to the development of a superior cultivar 'Maris Haplona'. In bell peppers, DH lines produced from anther derived haploids, exhibited all shades of colour ranging from dark green found in one parent to light green found in the other parent of the hybrid employed.

Despite the above conspicuous achievements made through haploid breeding, we find that on a world wide level, the acreage occupied by varieties developed through haploid breeding is negligible.

In India, no variety in any crop has so far been developed through haploid breeding. Even at an international level the emphasis on haploid breeding has gone down in recent years, partly due to failure in achieving the expected targets and partly due to shift in emphasis towards plant breeding based on molecular mapping.