Ovule Culture - Haploid have also been successfully produced from cultured female gametophytes. Success in this connection was first achieved with some Gymnosperms like Zamia, Ephedra and some Cycads.

Although initial attempts for the production of haploids from cultured ovules in an giosperms were unsuccessful, recently haploids could be obtained from cultured ovaries in a few crop species, like barley, wheat and tobacco. With more efforts in the future, haploids from ovule culture will be produced in a number of plant species. Further research is, therefore, necessary to improve our understanding about the following;

(i) the events leading to induction of haploidy in female gametophytes,