Pollen Culture - The technique of anther culture suffers from one main disadvantage, Le., plants may, originate not only from the pollen grains, but also from various parts of the anther, with the result that a mixed population of plants with various levels of ploidy may be obtained in a given culture.

These plantlets of higher ploidy originating from the diploid tissue cannot be utilized for production of true breeding lines though they may have other uses. In addition, the anther wall may also have deleterious effects on the development of pollen grains into embryos.