Advancements
OR
Improvements
in
Hybridoma
Technology -
Considerable
efforts
during
the
last
10-15
years
have
been
made
to
improve
the
yield
of
monoclonal
antibodies
using
hybridoma
technology.
These
efforts
included
the
following:
(i)
As
indicated
above,
cell
fusions
were
facilitated
through
the
use
of
polyethylene
glycol
(PEG).
(ii)
A
continuous
cell
line
(Sp
2/0)
was
used
as
a
fusion
partner
for
the
antibody
producing
B
cells.
(iii)
Feeder
layers
consisting
of
extra
cells
to
feed
newly
formed
hybridomas
were
used
for
optimal
growth
and
hybridoma
production;
