Affinity Chromatography - In affinity chromatography, the monoclonal antibody is generally immobilized on an insoluble matrix such as cross linked dextran or agarose beads, by a covalent linking agent (e.g. cyanogens bromide).

The immobilized antibody is generally packed in a Column, where its average concentration is 5-10mg antibody per ml of bed volume.

Protein solution containing the specific enzyme is applied over these immobilized antibodies. The specific enzyme binds with the antibody while other components do not, and thus the specific enzyme is separated.