Purification
of
Antibodies -
Monoclonal
antibodies
may
need
to
be
purified
before
they
are
used
for
a
variety
of
purposes.
Before
final
purification,
the
cultures may
be
subjected
to
cell
fractionation
for
enrichment
of
the
antibody
protein.
In
E.
coli,
the
antibodies
may
be
secreted
in
the
periplasm,
which
may
be
used
for
enrichment
of
antibody,
so
that
further
purification
is
simplified.
Alternatively
the
antibodies
may
be
purified
from
cell
homogenate
or
cell
debris
obtained
from
the
medium.
Antibodies
can
be
purified
by
anyone
of
the
following
techniques
(i)
ion-exchange
chromatography;
(ii)
antigen
affinity
chromatography.
